Last edited by Yozshuk
Monday, May 18, 2020 | History

3 edition of Bacterial Artificial Chromosomes (Methods in Molecular Biology (Clifton, N.J.), V. 255-256.) found in the catalog.

Bacterial Artificial Chromosomes (Methods in Molecular Biology (Clifton, N.J.), V. 255-256.)

  • 90 Want to read
  • 8 Currently reading

Published by Humana Pr .
Written in English

    Subjects:
  • General,
  • Science,
  • Chromosomes, Artificial, Bacterial,
  • Biotechnology,
  • Medical,
  • Laboratory manuals,
  • Cloning, Molecular,
  • Genetics (non-medical),
  • Molecular biology,
  • Bacterial artificial chromosomes,
  • Bacterial artificial chromosom,
  • Chromosome Mapping,
  • Chromosomes, Artificial, Bacte

  • Edition Notes

    ContributionsShaying Zhao (Editor), Marvin Stodolsky (Editor)
    The Physical Object
    FormatHardcover
    Number of Pages308
    ID Numbers
    Open LibraryOL9784154M
    ISBN 10159259753X
    ISBN 109781592597536

    Bacterial Artificial Chromosomes (BAC) have been developed to accommodate much larger pieces of DNA than plasmids can. To provide maximum information and utility, a BAC library is generally organized into a map that reflects the DNA sequence in a chromosome. bacterial artificial chromosome (BAC) CLONING VECTOR based on the F PLASMID of ESCHERICHIA COLI, with the capacity to accept DNA inserts generally –kb in size. Often used in the CLONE CONTIG SEQUENCING METHOD to obtain sequence CONTIGS in a genome sequencing project.

      1. Nature. Oct 30;() A gene expression atlas of the central nervous system based on bacterial artificial chromosomes. Gong S(1), Zheng C, Doughty ML, Losos K, Didkovsky N, Schambra UB, Nowak NJ, Joyner A, Leblanc G, Hatten ME, Heintz by:   A bacterial artificial chromosome (BAC) is one of a class of tools, called vectors, that microbiologists use to insert genes into a bacterium — usually e ing genes alters the properties of the bacterium in a process called transformation.

    This animation takes you through the techniques used to make a BAC library, an essential process during the Human Genome Project.. A Bacterial Artificial Chromosome (BAC) acts as a vehicle to artificially carry and store DNA into a bacterial cell. Because the human genome is too large to be sequenced from one end to the other in one go it has to be split up into manageable chunks.   Bacterial Artificial Chromosomes - Science topic BAC is a DNA construct, based on a functional fertility plasmid (or F-plasmid), used for transforming and cloning in bacteria, usually E. coli.


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Bacterial Artificial Chromosomes (Methods in Molecular Biology (Clifton, N.J.), V. 255-256.) Download PDF EPUB FB2

Bacterial artificial chromosomes (BACs) are plasmids used for cloning and stably maintaining large segments of foreign DNA in Escherichia is important in various types of analyses of mammalian and other genomes. A problem in some recombinant DNA experiments is the stable maintenance of large (> kilobase pairs) inserts in E.

coli. Bacterial Artificial Chromosomes, Second Edition expands upon the previous edition with current, detailed methods developed for working with d chapters included in this edition present fundamental techniques used Bacterial Artificial Chromosomes book BAC construction and characterization, advanced procedures for introducing modifications, achieving gene expression from BAC vectors, applications of BACs in model.

BAC vectors (Bacterial Artificial Chromosome) The F (fertility) factor is a plasmid that can be mobilized from F+ male bacteria and F- female bacteria. The gene transfer from one to another bacterial cell is called conjugation. The F factor controls its own replication. About this book The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine.

In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of. Bacterial Artificial Chromosomes, Second Edition expands upon the previous edition with current, detailed methods developed for working with BACs.

Updated chapters included in this edition present fundamental techniques used for BAC construction and characterization, advanced procedures for introducing modifications, achieving gene expression from BAC vectors, applications of BACs in model.

The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs.

Yeast artificial chromosomes also may produce chimeric effects, while bacteria artificial chromosomes will not. The usage of artificial chromosomes is mainly for studying DNA Bacterial Artificial Chromosomes book. This is done by integrating a non-viral/non-bacterial DNA into a bacterial chromosome and having it express the DNA fragment within the host.

Bacterial artificial chromosome (BAC). Bacterial artificial chromosomes (BACs) involve a cloning system that is derived from a particular plasmid found in the bacterium Escherichia use of the BAC allows large pieces of deoxyribonucleic acid (DNA) from bacterial or non-bacterial sources to be expressed in Escherichia ed expression of the foreign DNA produces many copies in.

Get this from a library. Bacterial artificial chromosomes. [Shaying Zhao; Marvin Stodolsky;] -- Describes the methods for transferring and maintaining DNA within and between species, presented in step-by-step format. Includes scientific reviews, software tools, database resources and sequencing.

A free online edition of this book is available at Additional hard copies can be obtained from [email protected] Bacterial Artificial Chromosomes, Edited by Pradeep Chatterjee p. ISBN Product Information.

This book gives emphasis to the various applications of Bacterial Artificial Chromosomes (BACs) in various studies. The topics presented are - using BAC libraries as assets for the mapping of marsupial and monotreme gene and for comparative genomic studies, and the usage of BACs as vehicles for maintaining the large infectious DNA genomes of viruses.

(source: Nielsen Book Data) Summary Bacterial Artificial Chromosomes provides a comprehensive collection of the protocols and resources developed for BACs in recent years. These two volumes collectively cover four topics about BACs: 1) library construction, 2) physical mapping, 3) sequencing, and 4) functional studies.

The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant : Paperback.

A supplemental appendix is to a book as a _____ is to a bacterial chromosome. plasmid. Which of the following vectors should be used. bacterial artificial chromosomes. What genetic information does cDNA contain. the DNA sequence of expressed genes.

An advantage of using reverse transcriptase to prepare a gene for cloning is that. The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant : Hardcover.

Bacterial artificial chromosome (BAC) vectors are important tools for microbial genome research. We constructed a novel BAC vector, pUvBBAC, for replication in both gram-negative and gram-positive bacterial hosts.

The pUvBBAC vector was used to generate a BAC library for the facultative intracellular pathogen Listeria monocytogenes EGD-e.

The library had insert sizes ranging from 68 to Cited by: Many bacteria (and some yeasts or other fungi) also possess looped bits of DNA known as plasmids, which exist and replicate independently of the ds have relatively few genes (fewer than 30). The genetic information of the plasmid is usually not essential to survival of the host bacteria.

Bacterial Artificial Chromosomes (BAC): Bacterial artificial chromosomes (BAC) were developed by Mel Simmons and coworkers in the early s and are based on the fertility factor (F factor) of Escherichia coli.

The F plasmid, a ~ kb circular double stranded DNA, is. A human artificial chromosome (HAC) is a microchromosome that can act as a new chromosome in a population of human cells. That is, instead of 46 chromosomes, the cell could have 47 with the 47th being very small, roughly 6–10 megabases (Mb) in size instead of 50– Mb for natural chromosomes, and able to carry new genes introduced by human researchers.

/ Site-specific integration of bacterial artificial chromosomes into human cells. Bacterial Artificial Chromosomes. editor / Kumaran Narayanan. Vol. 2nd. NY USA: Springer, pp.

(Methods in Molecular Biology).Author: Bradley McColl, Bradley McColl, Sara Howden, Sara Howden, Jim Vadolas, Jim Vadolas. Yeast artificial chromosomes (YACs) provide the largest insert capacity of any cloning system.

Yeast expression vectors, such as YACs, YIPs (yeast integrating plasmids), and YEPs (yeast episomal plasmids), have advantageous over bacterial artificial chromosomes (BACs).PDF | OnHope M. Wolf and others published Functionalizing Bacterial Artificial Chromosomes with Transposons to Explore Gene Regulation | Find, read and cite all the research you.- Yeast artificial chromosomes correspond to the top tools for the learning of eukaryotic genomes and for mobilisation of huge genetic elements among bacteria and eukaryotes.

- YACs function like wildiy existing chromosomes, in regard that they are of the appropriate size, revealing analogous stability.